Nucleoside and Nucleotide Analogues: Chemistry, Manufacturing and CMC Strategies for Regulatory Submission

Scientific background and therapeutic context Nucleosides, comprising a nitrogenous base covalently linked to ribose or deoxyribose, are fundamental precursors for nucleic acid biosynthesis and form the chemical core of oligonucleotide monomers used in solid‑phase synthesis. Chemically manufactured DNA phosphoramidites typically contain a protected 5′‑hydroxyl (5′‑DMT) and a 3′‑phosphoramidite bearing cyanoethyl and diisopropylamine moieties, while RNA […]